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HSP27 Antibody Phospho (pS15)

Rabbit Monoclonal Antibody

  • WB - HSP27 Antibody Phospho (pS15) AJ1378b
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-HSP27 (pS15) RabMAb (Cat. #AJ1378b), 1:5000 dilution. Cells were either (A) untreated (B) treated with heat at 44 oC
  • IF - HSP27 Antibody Phospho (pS15) AJ1378b
    B. Immunofluorescent staining of HeLa cells using anti-phospho HSP27 (pS15) RabMAb (Cat. #AJ1378b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04792
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP2293Y
Calculated MW 22783 Da
Gene ID 3315
Other Names Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Serine 15 of human HSP27 was used as immunogen. The antibody will detect HSP27 phosphorylation on Serine 15.
Dilution WB~~1:2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSP27 Antibody Phospho (pS15) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPB1
Synonyms HSP27, HSP28
Function Involved in stress resistance and actin organization.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles
Tissue Location Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle
Research Areas
Citations (0)

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Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).


1. Mendelsohn, M.E., et al.. Proc Natl Acad Sci U S A. 88: 11212
2. Faucher,C.,et al. J.Biol Chem.268:15168
3. Landry, J., et al. J. Biol. Chem. 267: 794
4. Rouse, J., et al. Cell 78: 1027-1037 (1994).

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Cat# AJ1378b
(40 western blots)
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