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HSP27 Antibody Phospho (pS78)

Rabbit Monoclonal Antibody

  • WB - HSP27 Antibody Phospho (pS78) AJ1378c
    A. Western blot analysis on A431 cell lysates using anti- Phospho-HSP27 (pS78) RabMAb (Cat. #AJ1378c), 1:2,000 dilution. Cells were either (A) untreated (B) treated with Anisomycin
  • IHC - HSP27 Antibody Phospho (pS78) AJ1378c
    B. Immunohistochemical analysis of paraffin embedded human bhreast carcinoma using anti-Phospho-HSP27 (pS78) RabMAb (Cat. #AJ1378c)
  • IF - HSP27 Antibody Phospho (pS78) AJ1378c
    C. Immunofluorescent staining of HeLa cells using anti-Phospho-HSP27 (pS78) (Cat. #AJ1378c).
  • DB - HSP27 Antibody Phospho (pS78) AJ1378c
    D. Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti- Phospho-HSP27 (pS78) RabMAb (Cat. #AJ1378c) dilution 1:2,000
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04792
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names Y175
Calculated MW 22783 Da
Gene ID 3315
Other Names Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Serine 78 of human HSP27 was used as immunogen. The antibody will detect Src phosphorylation on Serine 78.
Dilution WB~~1:2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSP27 Antibody Phospho (pS78) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPB1
Synonyms HSP27, HSP28
Function Involved in stress resistance and actin organization.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles
Tissue Location Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle
Research Areas
Citations (0)

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Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).


1. Mendelsohn, M.E., et al. The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein. Proc Natl Acad Sci U S A. 88: 11212
2. Faucher,C.,et al. The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein.J.Biol Chem. 268:15168
3. Landry, J., et al. Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II. J. Biol. Chem. 267: 794
4. Rouse, J., et al. A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins. Cell 78: 1027

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Cat# AJ1378c
(40 western blots)
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