|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||48841 Da|
|Other Names||Serine protease HTRA2, mitochondrial, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HTRA2, OMI, PRSS25|
|Target/Specificity||A synthetic peptide corresponding to N-terminal residues of mature form of human HtrA2 was used as immunogen. The antibody does not cross-react with other peptidase S1C family members.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HtrA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.|
|Cellular Location||Mitochondrion intermembrane space. Mitochondrion membrane; Single-pass membrane protein. Note=Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID|
|Tissue Location||Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid|
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Provided below are standard protocols that you may find useful for product applications.
HtrA2 (Omi) is a serine protease that plays a significant role in programmed cell death, or apoptosis. Localized to the intermembrane space of the mitochondria, HtrA2 exists predominantly as two isoforms (1). In response to cellular stress signals, HtrA2 is released into the cytosol. One way that HtrA2 induces apoptosis is by binding to BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), and thereby inhibiting their caspase-inhibitory activity (2,3).
1. Gray, C.W. et al. Characterization of human HtrA2, a novel serine protease involved in the mammalian cellular stress response. Eur. J. Biochem. 267: 5699
2. Savopoulos, J.W. et al. Expression, purification, and functional analysis of the human serine protease HtrA2. Protein Expr. Purif. 19: 227
3. Suzuki, Y. et al. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell 8: 613
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