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HtrA2 Antibody (N-term)

Rabbit Monoclonal Antibody

  • WB - HtrA2 Antibody (N-term) AJ1384b
    A. Western blot analysis on Jurkat cell lysate using anti-HtrA2 RabMAb (Cat. #AJ1384b), dilution 1:2,000.
  • IHC - HtrA2 Antibody (N-term) AJ1384b
    B. Immunohistochemical staining of paraffin-embedded human lung adenocarcinoma using anti-HtrA2 RabMAb (Cat. #AJ1384b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43464
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E55
Calculated MW 48841 Da
Gene ID 27429
Other Names Serine protease HTRA2, mitochondrial, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HTRA2, OMI, PRSS25
Target/Specificity A synthetic peptide corresponding to N-terminal residues of mature form of human HtrA2 was used as immunogen. The antibody does not cross-react with other peptidase S1C family members.
Dilution WB~~1:500~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHtrA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HTRA2
Synonyms OMI, PRSS25
Function Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
Cellular Location Mitochondrion intermembrane space. Mitochondrion membrane; Single-pass membrane protein. Note=Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID
Tissue Location Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid
Research Areas
Citations (0)

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HtrA2 (Omi) is a serine protease that plays a significant role in programmed cell death, or apoptosis. Localized to the intermembrane space of the mitochondria, HtrA2 exists predominantly as two isoforms (1). In response to cellular stress signals, HtrA2 is released into the cytosol. One way that HtrA2 induces apoptosis is by binding to BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), and thereby inhibiting their caspase-inhibitory activity (2,3).


1. Gray, C.W. et al. Characterization of human HtrA2, a novel serine protease involved in the mammalian cellular stress response. Eur. J. Biochem. 267: 5699
2. Savopoulos, J.W. et al. Expression, purification, and functional analysis of the human serine protease HtrA2. Protein Expr. Purif. 19: 227
3. Suzuki, Y. et al. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell 8: 613

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Cat# AJ1384b
(40 western blots)
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