|Application ||WB, IHC|
|Calculated MW||57825 Da|
|Other Names||Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human ICAM-1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ICAM-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Antigen-specific cell contacts in the immune system are strengthened by antigen-nonspecific interactions, mediated in part by lymphocyte-function associated (LFA) antigens. Recently, ICAM-1 (intercellular adhesion molecule-1) has been defined as a ligand for LFA-1. Monoclonal antibodies to ICAM-1 block T lymphocyte adhesion to fibroblasts and endothelial cells and disrupt the interaction between cytotoxic T cells and target cells. ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines (1). The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. However, ICAM-1 is also used as a receptor by the major group of human rhinoviruses and is a catalyst for the subsequent viral uncoating during cell entry (2). Monoclonal antibodies recognize a 95 kDa cell surface glycoprotein the major human rhinovirus receptor, ICAM-1 (3).
1. Simmons D, et al. Nature 331(6157):624-7, 1988.
2. Bella J, et al. Proc Natl Acad Sci USA 95(8)4140-5, 1998.
3. Greve JM, et al. Cell 56(5):839-47, 1989.
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