- CITATIONS: 1
|Application ||WB, FC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||63525 Da|
|Other Names||Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, CRF2-1, Type I interferon receptor 1, IFNAR1, IFNAR|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human IFN-α R1 was used as an immunogen. This antibody is specific for the 90kda precursor and the full-length of IFN-α R1 protein.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IFN-alpha R1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1 (PubMed:2153461, PubMed:7665574, PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:21854986). Functions in general as heterodimer with IFNAR2 (PubMed:7665574, PubMed:10049744, PubMed:21854986). Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta- subunits themselves (PubMed:7665574, PubMed:21854986). Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).|
|Cellular Location||Isoform 1: Cell membrane; Single- pass type I membrane protein. Late endosome Lysosome. Note=Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes.|
|Tissue Location||IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R|
Provided below are standard protocols that you may find useful for product applications.
Interferon alpha receptor 1 (IFN-? R1) is a class II cytokine receptor that belongs to the type I human interferons (IFNs) family. IFNs plays a role in antiviral, antiproliferative, immunomodulatory, antitumor, and antiparasitic activities by inducing transcription of IFN-stimuated genes (ISGs) through activation of the Jak-STAT pathway. Specifically, IFN- ? R1 is a 135-kDa signal transducing subunit of the IFN alpha/beta receptor complex (IFN-? R) (1). IFN-? R1 is phosphorylated through PTK- and PKC-mediated phosphorylation, which initiates docking of STAT proteins to IFNs. This leads to activation of STAT proteins by PTK-mediated phosphorylation. The mechanism behind the amplification of IFN-? R complex mediated transmembrane signaling has been linked to tyrosine phosphorylation on IFN-? R1 (2).
1. Kotenko SV, et al Nat Acad Sci USA, 96:5007-5012, 1999.
2. Constantinescu SN, et al Proc Natl Acad Sci USA 91:9602-9606, 1994.
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