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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   IkappaB-alpha Antibody Phospho (S32)   

IkappaB-alpha Antibody Phospho (S32)

Rabbit Monoclonal IgG

  • WB - IkappaB-alpha Antibody Phospho (S32) AJ1389d
    Western blot analysis on Hela cell lysates using anti-Phospho-IkappaB-alpha (pS32) RabMAb (Cat. #AJ1389d). Cells were either (A) untreated (B) treated with Calyculin A and TNF-a
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25963
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3148
Calculated MW 35609 Da
Gene ID 4792
Other Names NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Target/Specificity A phospho specific peptide corresponding to residues surrounding Serine 32 of human IkappaB-alpha was used as an immunogen.
Dilution WB~~1:100010000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIkappaB-alpha Antibody Phospho (S32) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms IKBA, MAD3, NFKBI
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Research Areas
Citations (0)

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The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein (1-5). In response to many different NF-kappaB-inducing agents including T-cell mitogens, proinflammatory cytokines, and viral transactivators, the inhibitory IkappaB-alpha is rapidly phosphorylated and degraded (2). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha (3). Activation of NF-kappaB can also be achieved after tyrosine phosphorylation of IkappaBalpha at tyrosine 42, an event that also ultimately leads to the dissociation of NF-kappaB and IkappaBalpha (4). Phosphorylation of IkappaB-alpha at Ser32 and Tyr42 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism (1-5).


1. Traenckner E.B., et al. EMBO J. 14: 2876-83, 1995 2. Brockman J.A., et al. Mol. Cell. Biol. 15(5): 2809-18, 1995 3. Chen Z.J., et al. Cell 84(6): 853-62, 1996 4. Witherow DS., et al. Proc Natl Acad Sci USA. 101(23):8603-8607, 2004 5. Brown K., et al. Science 267(5203): 1485-8, 1995

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Cat# AJ1389d
(40 western blots)
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