|Reactivity||Human, Mouse, Rat|
|Calculated MW||20517 Da|
|Other Names||Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human IL-10 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IL-10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.|
|Tissue Location||Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types|
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Provided below are standard protocols that you may find useful for product applications.
Interleukin (IL)-10 is a cytokine that inhibits production of other regulatory factors, including interferon gamma (IFN-gamma) and IL-2. A dimer of IL-10 is present in solution and is presumed to participate in receptor binding (1). IL-10 is a natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. Human IL-10 (hIL-10) has high homology with murine IL-10 (mIL-10) as well as with an Epstein-Barr virus genome product BCRFI (2). IL-10 displays pleiotropic immunoregulatory properties. Human and murine IL-10 have a high amino acid sequence identity (ca. 73%) which includes the conservation of all four cysteine residues in human IL-10 and the first four out of five cysteine residues for murine IL-10. IL-10 is a highly alpha-helical protein containing two disulfide bonds, either one or both of which are critical for its structure and function (3).
1. Windsor WT, et al. Biochemistry 32(34):8807-15, 1993.
2. Gesser B, et al. Proc Natl Acad Sci 94(26):14620-5, 1997.
3. Zdanov A, et al. Structure 3(6):591-601, 1995.
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