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IL-4 Antibody

Rabbit Monoclonal Antibody

     
  • WB - IL-4 Antibody AJ1399a
    A. Western blot analysis on Human tonsil lysate using anti-IL-4 RabMAb (Cat. #AJ1399a), dilution 1:2000.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P05112
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR1118Y
Calculated MW 17492 Da
Gene ID 3565
Other Names Interleukin-4, IL-4, B-cell stimulatory factor 1, BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, IL4
Target/Specificity A synthetic peptide corresponding to residues in human IL-4 was used as an immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL4
Function Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity).
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

Interleukin-4 (IL-4) is a principal regulatory cytokine during an immune response and a crucial determinant for allergy and asthma. IL-4 binds with high affinity and specificity to the ectodomain of the IL-4 receptor alpha chain (IL4-BP). IL-4 is a left-handed four-helix bundle with a short stretch of beta sheet. The structure bears close resemblance to other cytokines such as granulocyte-macrophage colony stimulating factor (GM-CSF). Regions have been identified which are not only important to maintain structure, but could also play a role in receptor binding (2). IL-4 antagonists Y124D and Y124G are presented and shown to be medically important. The site around Y124 is a particularly important epitope responsible for the ability of IL-4 to cause a signal in the target cells (3).

References

1. Hage T, et al. Cell 97(2):271-81, 1999
2. Wlodawer, A, et al. FEBS Lett 309(1):59-64, 1992
3. Muller T, et al. J Mol Biol 237(4):423-36, 1994

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Discontinued
Cat# AJ1399a
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