|Calculated MW||17492 Da|
|Other Names||Interleukin-4, IL-4, B-cell stimulatory factor 1, BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, IL4|
|Target/Specificity||A synthetic peptide corresponding to residues in human IL-4 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IL-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.|
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Provided below are standard protocols that you may find useful for product applications.
Interleukin-4 (IL-4) is a principal regulatory cytokine during an immune response and a crucial determinant for allergy and asthma. IL-4 binds with high affinity and specificity to the ectodomain of the IL-4 receptor alpha chain (IL4-BP). IL-4 is a left-handed four-helix bundle with a short stretch of beta sheet. The structure bears close resemblance to other cytokines such as granulocyte-macrophage colony stimulating factor (GM-CSF). Regions have been identified which are not only important to maintain structure, but could also play a role in receptor binding (2). IL-4 antagonists Y124D and Y124G are presented and shown to be medically important. The site around Y124 is a particularly important epitope responsible for the ability of IL-4 to cause a signal in the target cells (3).
1. Hage T, et al. Cell 97(2):271-81, 1999
2. Wlodawer, A, et al. FEBS Lett 309(1):59-64, 1992
3. Muller T, et al. J Mol Biol 237(4):423-36, 1994
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