|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||45251 Da|
|Other Names||Indian hedgehog protein, IHH, HHG-2, Indian hedgehog protein N-product, Indian hedgehog protein C-product, IHH|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human IHH was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Indian Hedgehog Antibody (IHH) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) (By similarity).|
|Cellular Location||Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side. Note=The N-terminal peptide remains associated with the cell surface. Cell membrane.|
|Tissue Location||Expressed in embryonic lung, and in adult kidney and liver|
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Provided below are standard protocols that you may find useful for product applications.
Hedgehog, WNT, FGF and BMP signaling pathways network together during embryogenesis, tissue regeneration, and carcinogenesis. Aberrant activation of Hedgehog signaling pathway leads to pathological consequences in a variety of human tumors, such as gastric cancer and pancreatic cancer (1). The hedgehog family of morphogens are regulators of cell proliferation, differentiation and cell-cell communication. These morphogens have been shown to have important roles in organogenesis, spermatogenesis, stem cell maintenance and oncogenesis. Indian hedgehog (IHH) has been shown to be expressed in the uterine epithelium under the control of the steroid hormone, progesterone (2). It has been shown that IHH, synthesized by prehypertrophic and hypertrophic chondrocytes, regulates the site of hypertrophic differentiation by signaling to the periarticular growth plate and also determines the site of bone collar formation in the adjacent perichondrium. By providing crucial local signals from prehypertrophic and hypertrophic chondrocytes to both chondrocytes and preosteoblasts, IHH couples chondrogenesis to osteogenesis in endochondral bone development (3).
1. Katoh Y, et al. Cancer Biol Ther 4(10):1050-4, 2005.
2. Lee K, et al. Nat Genet. 38(10):1204-9, 2006.
3. Chung UI, et al. J Clin Invest 107(3):295-304, 2001.
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