|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||87058 Da|
|Other Names||Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa, CD61, ITGB3, GP3A|
|Target/Specificity||A synthetic peptide corresponding to residues near C-terminus (the cytoplasmic region) of human Integrin β-3A was used as an immunogen. This antibody does not detect Integrin β-3B or Integrin β-3C.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Integrin beta-3A/CD61 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha- IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.|
|Cellular Location||Cell membrane; Single- pass type I membrane protein. Cell projection, lamellipodium membrane Cell junction, focal adhesion|
|Tissue Location||Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis|
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Provided below are standard protocols that you may find useful for product applications.
Platelet membrane glycoprotein Integrin β-3 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar membrane glycoprotein complex is present on endothelial cells. Homology suggests that GP IIIa is a member of a family of cell-surface adhesion receptors. (1) Data provide further support for the key role of the cytoplasmic domain of the beta3 integrin in cell adhesion and suggest a potential role for the beta3C integrin subunit in modulating cell-matrix interactions (2). It has been demonstrated that the beta 3 subunit of alpha IIb beta 3 was phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3).
1. Fitzgerald LA, et al. J Biol Chem 262(9):3936-9, 1987
2. Kumar CS, et al. J Biol Chem 272(26):16390-7, 1997
3. Law DA, et al. J Biol Chem 271(18):10811-5, 1996
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