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Integrin-alpha4 Antibody Phospho-ps1027Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1409a | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Integrin-alpha4 Antibody Phospho-ps1027 - Product info | |
| Application | WB
|
| Primary Accession | P13612 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EPR2343 |
| Calculated MW | 114900 Da |
| Gene ID 3676 | |
| Other Names ITGA4, CD49D, Integrin alpha-4, Integrin alpha-IV;VLA-4;CD49 antigen-like family member D | |
| Target/Specificity A phospho-specific peptide corresponding to residues on human Integrin alpha 4 was used as an immunogen. The antibody only detects Integrin alpha4 phosphorylated on Serine 1027. The antibody detects the mature chain (180 kDa), the precursor (140 kDa) and the cleaved C terminal (70 kDa). | |
| Dilution WB~~1:500~2000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Integrin-alpha4 Antibody Phospho-ps1027 is for research use only and not for use in diagnostic or therapeutic procedures. | |
Integrin-alpha4 Antibody Phospho-ps1027 - Protein Information | |
| Name ITGA4 | |
| Synonyms CD49D | |
| Function Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells | |
| Cellular Location Membrane; Single-pass type I membrane protein | |
Integrin-alpha4 Antibody Phospho-ps1027 - Application data
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A. Western blot analysis on THP-1 cell lysates using anti-Phospho-Integrin alpha 4 (pS1027) RabMAb (Cat. #AJ1409a), 1:2000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase
Integrin-alpha4 Antibody Phospho-ps1027 - Related products
RI12780: ITGA4 predesign siRNA
AT2564a: ITGA4 Antibody (monoclonal) (M01)
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BACKGROUND
Integrin alpha 4 is a cell surface heterodimer (VLA4, CD49d/CD29) which belongs to the integrin family and is involved in cell-extracellular matrix interactions, as well as in intercellular adhesion. Unlike other integrin alpha subunits, the alpha subunit of Integrin alpha 4 can appear as a 150-kDa polypeptide (alpha 150), or cleaved into two non-covalently associated polypeptides (alpha 70-80). The relative proportion of each form is highly variable among different cells and is dependent on the state of cellular activation (1). Integrin alpha 4 regulates cellular functions, differently from other integrins, through its cytoplasmic tail, making it indispensable for embryogenesis, haematopoiesis and immune responses (2). Integrin alpha 4, initially discovered on lymphoid cells, is actually known to be highly expressed on T cells, B cells, monocytes, and derived cell lines (3). It has a key function in the adhesion interaction between stem/progenitor cells and the stromal microenvironmental cells and their matrix within the bone marrow (4).
REFERENCES
1. Rubio M, et al. Eur J Immunol. 22(4):1099-102, 1992
2. Liu S, et al. Nature. 402(6762):676-81, 1999
3. Zhang ZH, et al. Blood. 78(9):2396-9, 1991
4. Mittelbrunn M, et al. UCSD-Nature Molecule Pages, 2006
