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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IPP-1 Antibody   

IPP-1 Antibody

Rabbit Monoclonal Antibody

     
  • WB - IPP-1 Antibody AJ1410a
    A. Western blot analysis on rat brain using anti-IPP-1 (N-term) RabMAb (Cat. #AJ1410a), dilution 1:40,000
  • IHC - IPP-1 Antibody AJ1410a
    B. Immunohistochemical analysis of paraffin-embedded human normal brain tissue using anti_IPP1 RabMAb. (Cat. #AJ1410a).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q13522
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP902Y
Calculated MW 19011 Da
Gene ID 5502
Other Names Protein phosphatase 1 regulatory subunit 1A, Protein phosphatase inhibitor 1, I-1, IPP-1, PPP1R1A, IPP1
Target/Specificity A synthetic peptide corresponding to residues in the N-terminus of human IPP-1 was used as an immunogen.
Dilution WB~~1:100000
IHC~~1:100250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIPP-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP1R1A
Synonyms IPP1
Function Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.
Research Areas
Citations (0)

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Background

The inhibitor of protein phosphatase 1 (IPP-1, I-1) is an endogenous inhibitor of protein phophastase 1 (PP-1), a signal transducer in many cellular pathways. IPP-1 is a member of heat-stable PP1 inhibitory subunits, which includes DARPP-32, (a homologue of IPP-1) and IPP-2. First discovered in rabbit skeletal muscle, IPP-1 is expressed in various mammalian tissues. IPP-1 inhibition of PP-1 is controlled through phosphorylation at Thr35 by cAMP-dependent protein kinase and a tetra-peptide consensus motif in various PP-1 binding proteins (1). IPP-1 is a hormonal control in muscle contraction, pituitary cell growth, synaptic plasticity by neurotransmitters, and glycogen metabolism (2). In vivo, IPP-1 is phosphorylated at Ser-67 by NCLK, which also initiates inhibition of PP-1 by IPP-1 (3). Triggered by increase in calcium level, IPP-1 is inactivated by PP2B through de-phosphorylation (4).

References

1. Egloff MD, et al. EMBO J 16:1876-1887, 1997.
2. Oliver CJ, Shenolikar S, Front Biosci 3:D961-972, 1998.
3. Huang K, Paudel HK, PNAS 97:5824-5829, 2000.
4. Castellani GC, Learn Mem 12:423-432, 2005.

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Discontinued
Cat# AJ1410a
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