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IRF3 Antibody Phospho (pS386)

Rabbit Monoclonal Antibody

  • WB - IRF3 Antibody Phospho (pS386) AJ1413c
    A.Western blot analysis on MCF-7 cell lysates using anti-Phospho-IRF3 (pS386) RabMAb (Cat. #AJ1413c), 1:20,000 dilution. Cells were either (A) untreated (B) treated with Calyculin A
  • IF - IRF3 Antibody Phospho (pS386) AJ1413c
    B.Immunofluorescent staining of MCF7 cells using Phospho-specific anti-IRF3 RabMAb (Cat. #AJ1413c).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14653
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR2346
Calculated MW 47219 Da
Gene ID 3661
Other Names Interferon regulatory factor 3, IRF-3, IRF3
Target/Specificity A phospho-specific peptide corresponding to residues surrounding Serine 386 of human IRF3 was used as an immunogen. The antibody only detects IRF3 phosphorylated on Serine 386.
Dilution WB~~1:2000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIRF3 Antibody Phospho (pS386) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IRF3
Function Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm
Tissue Location Expressed constitutively in a variety of tissues
Research Areas
Citations (0)

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A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) (1). IRF-3 is activated through phosphorylation by the kinases IKK epsilon and/or TBK1. Phosphorylation results in IRF-3 dimerization and removal of an autoinhibitory structure to allow interaction with the coactivators CBP/p300. Phosphorylation at Serine 396 through Serine 405 alleviates autoinhibition to allow interaction with CREB-binding protein and facilitates phosphorylation at Serine 385 or Serine 386 (2). Serine 386 is suggested to be the target of the IRF-3 kinase and critical determinant for the activation of IRF-3 (3).


1. Au, WC Proc Natl Acad Sci 92(25):11657-61, 1995
2. Panne D, et al. J Biol Chem. 282(31):22816-22, 2007
3. Mori M, et al. J Biol Chem. 279(11):9698-702, 2004

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Cat# AJ1413c
(40 western blots)
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