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ISG15 AntibodyRabbit Monoclonal
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1420b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
ISG15 Antibody - Product info | |
| Application | IF, WB
|
| Primary Accession | P05161 |
| Reactivity | Human |
| Clone Names | EPR3446 |
| Calculated MW | 17888 Da |
| Gene ID 9636 | |
| Target/Specificity A synthetic peptide corresponding to residues of human ISG15 was used as an immunogen. | |
| Dilution WB~~1:2000~5000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ISG15 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ISG15 Antibody - Protein Information | |
| Name ISG15 | |
| Synonyms G1P2, UCRP | |
| Function Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections | |
| Cellular Location Cytoplasm. Secreted. Note=UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted | |
| Tissue Location Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons | |
ISG15 Antibody - Related products
AP1150a: ISG15 Antibody (N-term)
AP1150b: ISG15 Antibody (C-term)
AP1150c: ISG15 Antibody (Center R87)
AP3721a: Phospho-PUM2- S182 Antibody
AP3733a: Phospho-PUM2- S136 Antibody
AP7569b: PUM2 Antibody (N-term)
BP1150a: ISG15 Antibody (N-term) Blocking Peptide
BP1150b: ISG15 Antibody (C-term) Blocking Peptide
BP1150c: ISG15 Antibody (Center R87) Blocking Peptide
BP3721a: Phospho-PUM2-S182 Antibody Blocking Peptide
BP3733a: Phospho-PUM2-S136 Antibody Blocking peptide
BP5827a: PUM2-S136 Antibody Blocking peptide
ISG15 Antibody - Application data
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Immunofluorescent staining of HeLa cells using anti-ISG15 RabMAb (Cat. #AJ1420b).
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Western blot analysis on (A) HeLa treated with IFN-a and (B) Daudi cell lysates using anti-ISG15 RabMAb (Cat. #AJ1420b).
ISG15 Antibody - Research Areas
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BACKGROUND
ISG15 is an ubiquitin like protein that is covalently conjugated to cellular proteins after IFN-alpha/beta stimulation (2). ISG15 is conjugated to their targets by an enzymatic cascade involving an E1 activating enzyme, an E2 conjugating enzyme, and in some cases an E3 ligase. ISG15 conjugation plays an important role by impacting nuclear and cytoplasmic functions as well as in antiviral response because a human virus, influenza B virus, inhibits ISG15 conjugation. However, the biological function of ISG15 modification remains unknown, largely because only a few human ISG15 target proteins have been identified (3).
REFERENCES
1. Zhao C, et al. Proc Natl Acad Sci 101(2):7578-82, 2004 2. Zhao C, et al. Proc Natl Acad Sci 102(29):10200-5, 2005
