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JAK2 Antibody Phospho (pY1007/1008)

Rabbit Monoclonal Antibody

  • WB - JAK2 Antibody Phospho (pY1007/1008) AJ1421a
    A. Western blot analysis of immunoprecipitation pellets from Jurkat cell lysate +GM-CSF (Cat. #AJ1421a), dilution 1:50.
  • IHC - JAK2 Antibody Phospho (pY1007/1008) AJ1421a
    B. Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using anti-JAK-2 (pY1007/1008) RabMAb (Cat. #AJ1421a).
  • DB - JAK2 Antibody Phospho (pY1007/1008) AJ1421a
    C. Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Jak2 (pY1007/1008) RabMAb (Cat. #AJ1421a) dilution 1:1,000
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60674
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E132
Calculated MW 130674 Da
Gene ID 3717
Other Names Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2, JAK2
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and Tyr1008 of human JAK2 was used as immunogen. The antibody only detects JAK2 phosphorylated on Tyrosine 1007 and 1008.
Dilution WB~~1:5000~200000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsJAK2 Antibody Phospho (pY1007/1008) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name JAK2
Function Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Cellular Location Endomembrane system; Peripheral membrane protein. Cytoplasm. Nucleus
Tissue Location Ubiquitously expressed throughout most tissues.
Research Areas
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Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).


1. Leonard, W.J. and J.J. O
2. O'Shea, J.J., et al. Advances in the understanding of cytokine signal transduction: the role of Jaks and STATs in immunoregulation and the pathogenesis of immunodeficiency. J Clin Immunol. 17: 431
3. Gauzzi, M.C., et al. Interferon-alpha-dependent activation of Tyk2 requires phosphorylation of positive regulatory tyrosines by another kinase. J. Biol. Chem. 271: 20494
4. Peeters, P., et al. Fusion of TEL, the ETS-variant gene 6 (ETV6), to the receptor-associated kinase JAK2 as a result of t(9;12) in a lymphoid and t(9;15;12) in a myeloid leukemia. Blood 90: 2535

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Cat# AJ1421a
(40 western blots)
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