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Ki-67 Antibody

Rabbit Monoclonal Antibody

  • IHC - Ki-67 Antibody AJ1427a
    A.Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Ki-67 RabMAb. Note nuclear staining of proliferating cells (Cat. #AJ1427a).
  • IF - Ki-67 Antibody AJ1427a
    B. Immunofluorescent staining of HeLa cells using anti-Ki-67 RabMAb (Cat. #AJ1427a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P46013
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3611
Calculated MW 358694 Da
Gene ID 4288
Other Names Antigen KI-67, MKI67
Target/Specificity A synthetic peptide corresponding to residues in human Ki-67 was used as an immunogen.
Dilution IHC~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKi-67 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MKI67
Function Thought to be required for maintaining cell proliferation.
Cellular Location Nucleus. Nucleus, nucleolus. Chromosome. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes
Research Areas
Citations (0)

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Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells that are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in nuclear matrix in the later phases (1). Ki-67 is thought to be involved in the maintaining of cell proliferation, however, its exact mechanism for function is unknown. Due to the fact that Ki-67 can be used to determine the growth fraction (the proportion of the cells committed to the cell cycle), it is considered to be an important marker in human tumor diagnostics and prognostics (2).


1. Gerdes J Semin Cancer Biol 1(3):199-206, 1990.
2. Gerdes J, Scholzen T J Cell Physiol 182(3):311-322, 2000.

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Cat# AJ1427a
(40 western blots)
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