|Application ||WB, IF|
|Calculated MW||70703 Da|
|Other Names||Lamin-B receptor, Integral nuclear envelope inner membrane protein, LMN2R, LBR|
|Target/Specificity||A synthetic peptide corresponding to residues of human Lamin B Receptor was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Lamin B Receptor Antibody Receptor is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Anchors the lamina and the heterochromatin to the inner nuclear membrane.|
|Cellular Location||Nucleus inner membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization (1). Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures (2). The cleavage of lamins results in nuclear disregulation and cell death (3,4).
1. Gruenbaum, Y., et al. Review: nuclear lamins
2. Ye, Q. and H.J. Worman. Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane. J. Biol. Chem. 269: 11306
3. Oberhammer, F.A., et al. Chromatin condensation during apoptosis is accompanied by degradation of lamin A+B, without enhanced activation of cdc2 kinase. J. Cell Biol. 126: 827
4. Rao, L., et al. Lamin proteolysis facilitates nuclear events during apoptosis. J. Cell Biol. 135: 1441
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