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LAT Antibody Phospho (pY200)

Rabbit Monoclonal Antibody

  • WB - LAT Antibody Phospho (pY200) AJ1434c
    A. Western blot analysis on Jurkat cell lysates using anti-phosho LAT (pY200) RabMAb (Cat. #AJ1434c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43561
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP983(2)Y
Calculated MW 27930 Da
Gene ID 27040
Other Names Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein, pp36, p36-38, LAT
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 200 of human LAT (long isoform) was used as immunogen. The antibody only detects LAT phosphorylated on Tyrosine 200 of long isoform and Tyrosine 171 of short isoform.
Dilution WB~~1:5000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLAT Antibody Phospho (pY200) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LAT
Function Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
Cellular Location Cell membrane; Single-pass type III membrane protein Note=Present in lipid rafts
Tissue Location Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
Research Areas
Citations (0)

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LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 (on the short isoform of LAT) are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1. Tyr200 and Tyr220 on long isoform of LAT correspond to Tyr171 and Tyr191 on short isoform respectively (3).


1. Weber, J.R., et al. J Exp Med. 187: 1157
2. Zhang, W., et al. Cell 92: 83
3. Paz, P.E., et al. Biochem J. 356(Pt 2): 461-71 (2001).

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Cat# AJ1434c
(40 western blots)
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