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LCAT Antibody

Rabbit Monoclonal Antibody

  • WB - LCAT Antibody AJ1435a
    A. Western blot analysis on human serum using anti-LCAT RabMAb (Cat. #AJ1435a), dilution 1:10,000.
  • IHC - LCAT Antibody AJ1435a
    B. Immunohistochemical staining of paraffin-embedded human brain using anti-LCAT RabMAb (Cat. #AJ1435a).
  • IF - LCAT Antibody AJ1435a
    C. Immunofluorescent staining of HeLa cells using anti-LCAT RabMAb (Cat. #AJ1435a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04181
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR1384Y
Calculated MW 48535 Da
Gene ID 4942
Other Names Ornithine aminotransferase, mitochondrial, Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, Ornithine aminotransferase, hepatic form, Ornithine aminotransferase, renal form, OAT
Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human LCAT was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLCAT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OAT
Cellular Location Mitochondrion matrix
Research Areas
Citations (0)

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The enzyme, lecithin cholesterol acyltransferase (LCAT), is responsible for the esterification of plasma cholesterol mediating the transfer of an acyl group from lecithin to the 3-hydroxy group of cholesterol (1). Esterification of cholesterol in plasma is mediated by LCAT. In patients with familial LCAT deficiency only trace amounts or no LCAT protein is found. Heterozygotes for this disorder have approximately half the normal amount of the enzyme. LCAT reactivity is essential for normal lipoprotein metabolism and for a proper equilibrium between tissue and plasma cholesterol (2). Cholesterol transport in circulation and its removal from tissues depends on the activity of lecithin cholesterol LCAT. LCAT is a soluble enzyme that converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lyso-phosphatidylcholines on the surface of high-density lipoproteins (3).


1. Taramelli R, et al. Hum Genet 85(2):195-9, 1990.
2. Frohlich J, et al. Clin Biochem 15(6):269-78, 1982.
3. Jonas A, Biochem Biophys Acta 1529(1-3):245-56, 2000.

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Cat# AJ1435a
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