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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   LDH-B Antibody   

LDH-B Antibody

Rabbit Monoclonal Antibody

     
  • WB - LDH-B Antibody AJ1438a
    A. Western blot analysis on HeLa cell lysate using anti-LDH-B RabMAb (Cat. #AJ1438a).
  • IHC - LDH-B Antibody AJ1438a
    B. Immunohistochemical staining of paraffin-embedded human heart muscle using anti-LDH-B RabMAb (Cat. #AJ1438a).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P07195
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1565Y
Calculated MW 36638 Da
Gene ID 3945
Other Names L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46, LDHB
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human LDH-B was used as an immunogen.
Dilution WB~~1:1000~10000
IHC~~1:100~250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLDH-B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LDHB
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).

References

1. Kopperschlager G et al. J Chromatogr B Biomed Appl. 684(1-2):25-49, 1996.
2. Rodriguez S et al. Oncogene 22(12): 1880-1891, 2003

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Discontinued
Cat# AJ1438a
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