|Application ||WB, IHC|
|Calculated MW||36311 Da|
|Other Names||L-lactate dehydrogenase C chain, LDH-C, Cancer/testis antigen 32, CT32, LDH testis subunit, LDH-X, LDHC, LDH3, LDHX|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human LDH-C was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LDH-C Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Possible role in sperm motility.|
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Provided below are standard protocols that you may find useful for product applications.
Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa) (2). LDHA and LDHB are expressed in most somatic tissues, while expression of LDHC is confined to the germinal epithelium of the testes (3).
1. Kopperschlager G et al. J Chromatogr B Biomed Appl. 684(1-2):25-49, 1996.
2. Rodriguez S et al. Oncogene 22(12): 1880-1891, 2003
3. Edwards YH, et al. Dev Genet. 8(4):219-32, 1987
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