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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   LFA-beta2 (ITGB2) Antibody Phospho (pS745)   

LFA-beta2 (ITGB2) Antibody Phospho (pS745)

Rabbit Monoclonal Antibody

     
  • WB - LFA-beta2 (ITGB2) Antibody Phospho (pS745) AJ1443b
    A. Western blot analysis on Jurkat cell lysates using anti-Phospho-LFA-beta2 (pS745) RabMAb (Cat. #AJ1443b), 1:500 dilution. Cells were either (A) untreated (B) treated with AP.
  • IHC - LFA-beta2 (ITGB2) Antibody Phospho (pS745) AJ1443b
    B. Immunohistochemical analysis of human tonsils using anti-Phospho- LFA-beta2 (pS745) RabMAb (Cat. #AJ1443b).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P05107
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1288Y
Calculated MW 84782 Da
Gene ID 3689
Other Names Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Complement receptor C3 subunit beta, CD18, ITGB2, CD18, MFI7
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser745 of human LFA-β2 was used as immunogen. The antibody only detects LFA-β2 phosphorylated on Serine 745.
Dilution WB~~1:5000~10000
IHC~~1:250~500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLFA-beta2 (ITGB2) Antibody Phospho (pS745) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ITGB2
Synonyms CD18, MFI7
Function Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Leukocytes..
Research Areas
Citations (0)

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Background

In the immune system, integrins have essential roles in leukocyte trafficking and function. These include immune cell attachment to endothelial and antigen-presenting cells, cytotoxicity, and extravasation into tissues (1). Integrin adhesion receptors transduce signals that control complex cell functions which require the regulation of gene expression, such as proliferation, differentiation and survival. Their intracellular domain has no catalytic function, indicating that interaction with other transducing molecules is crucial for integrin-mediated signaling. JAB1 (Jun activation domain-binding protein 1), a coactivator of the c-Jun transcription factor, has been identified as a protein that interacts with the cytoplasmic domain of the beta2 subunit of the alphaL/beta2 integrin LFA-1 (2). Patients with leukocyte adhesion molecule (CD11/CD18, beta 2 integrins) deficiency have structural defects in the common beta subunit (CD18), which prevent heterodimer formation and normal cell surface expression of these receptors, leading to life-threatening bacterial infections (3).

References

1. Kellersch B, et al. Results Prob Cell Differ 43:245-57, 2006
2. Bianchi E, et al. Nature 404(6778):617-21, 2000
3. Nelson C, et al. J. Biol Chem 267(5):3351-7, 1992.

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Discontinued
Cat# AJ1443b
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