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LIMK2 Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - LIMK2 Antibody (C-term) AJ1444a
    A. Western blot analysis on Ramos membrane using anti-LIMK2 RabMAb (Cat. #AJ1444a), dilution 1:2000.
  • IHC - LIMK2 Antibody (C-term) AJ1444a
    B. Immunohistochemical staining of paraffin-embedded human ovary carcinoma using anti-LIMK2 RabMAb (Cat. #AJ1444a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P53671
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP969Y
Calculated MW 72232 Da
Gene ID 3985
Other Names LIM domain kinase 2, LIMK-2, LIMK2
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human LIMK2 was used as an immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLIMK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIMK2
Function Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Cellular Location Isoform LIMK2a: Cytoplasm. Nucleus. Note=Isoform LIMK2a is distributed in the cytoplasm and the nucleus
Tissue Location Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form In adult lung, both LIMK2a and LIMK2b is nearly equally observed
Research Areas
Citations (0)

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LIM-kinase 2 (LIMK2), a 75-kDa member of the LIM kinase family, is responsible for regulating actin polymerization via phosphorylation and inactivation of the actin depolymerizing factors ADF/cofilin (1). LIMK2 protein is found in the cytoplasm during interphase, redistributed to the mitotic spindle and then to the spindle midzone during anaphase to telophase (2). It has been shown that ROCK, a downstream protein kinase of Rho, activates LIMK2 by directly phosphorylates threonine 505 residue. Since activity of LIMK2 is cell cycle dependant, LIMK2 activity is markedly increased when HeLa cells are synchronized at mitosis with nocodazole treatment (3).


1. Sumi T, et al. J Biol Chem 276(1):670-76, 2001
2. Acevedo K, et al. J Histochem Cytochem 54(5): 487-501, 2006
3. Sumi T, et al. Exp Cell Res 312(7):1021-30, 2006

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Cat# AJ1444a
(40 western blots)
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