|Application ||WB, IHC, IF|
|Calculated MW||98664 Da|
|Other Names||Phosphatidate phosphatase LPIN1, Lipin-1, LPIN1, KIAA0188|
|Target/Specificity||A synthetic peptide corresponding to residues in human Lipin-1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Lipin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity).|
|Cellular Location||Nucleus membrane. Cytoplasm, cytosol. Endoplasmic reticulum membrane|
|Tissue Location||Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.|
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Provided below are standard protocols that you may find useful for product applications.
Lipin-1 is a Mg(2)+-dependent phosphatidate phosphatase (PAP) enzyme which catalyzes the conversion of phosphatidate to diacylglycerol, a key reaction in glycerolipid biosynthesis (1). It plays critical roles in controlling energy metabolism, has establishing the adipogenic gene expression program during early stages of differentiation, and in triglyceride accumulation in mature adipocytes (2, 3). Lipin-1 also functions as a transcriptional coactivator in conjunction with members of the peroxisome proliferator-activated receptor (PPAR) family (2). There are two isoforms of Lipin-1 (alpha and beta) which appear to serve distinct functions in adipocytes, with unique expression dynamics, subcellular localization, and effects on gene expression. Lipin-1B is the predominant form in mature adipocytes. Lipin-1 expression levels are associated with insulin sensitivity, metabolic rate, hypertension, and risk for the metabolic syndrome (4).
1. Peixiang Zhang, et al. Journal of Lipid Research 49: 1519-1528, 2008 2. Donkor J, et al. J Biol Chem. 2009 3. Croce MA, et al. Diabetes. 56(9):2395-9, 2007 4. Reue K., et al. FEBS Lett. 582(1):90-6, 2008
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