|Application ||WB, IHC|
|Calculated MW||31555 Da|
|Other Names||Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein, PKCSL, MARCKS, MACS, PRKCSL|
|Target/Specificity||A synthetic peptide corresponding to residues on human MARCKS was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARCKS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.|
|Cellular Location||Cytoplasm, cytoskeleton. Membrane; Lipid-anchor|
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Provided below are standard protocols that you may find useful for product applications.
The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion; in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). MARCKS) binds calmodulin and is a predominant, specific substrate of PKC which is phosphorylated during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion; it is redistributed from plasma membrane to cytoplasm when phosphorylated and is involved in leukocyte motility. It has been reported that MARCKS is a filamentous (F) actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).
1. Harlan DM, et al. J Biol Chem 266(22):14399-405, 1991
2. Hartwig JH, et al. Nature 356(6370):618-22, 1992.
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