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MARK3 Antibody

Rabbit Monoclonal Antibody

  • WB - MARK3 Antibody AJ1460a
    A. Western blot analysis on HeLa cell lysate using anti-MARK3 RabMAb (Cat. #AJ1460a), dilution 1:2000
  • IHC - MARK3 Antibody AJ1460a
    B. Immunohistochemical staining of paraffin-embedded human urinary bladder using anti-MARK3 RabMAb (Cat. #AJ1460a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27448
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EPR633Y
Calculated MW 84489 Da
Gene ID 4140
Other Names MAP/microtubule affinity-regulating kinase 3, C-TAK1, cTAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, EMK-2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Par-1a, Serine/threonine-protein kinase p78, MARK3, CTAK1, EMK2
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human MARK3 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMARK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MARK3
Synonyms CTAK1, EMK2
Function Involved in the specific phosphorylation of microtubule- associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus.
Cellular Location Cell membrane; Peripheral membrane protein
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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MARK3 is a dual-specificity protein kinase that controls entry into mitosis by dephosphorylating Cdc2 on both threonine 14 and tyrosine 15. MARK3 is phosphorylated on serine 216 throughout interphase but not during mitosis. Serine 216 phosphorylation mediates the binding of 14-3-3 protein to MARK3, and MARK3/14-3-3 complexes are present throughout interphase but not during mitosis (1). Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (2). MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signaling, indicating a link between two important developmental pathways (3).


1. Peng CY, et al Cell Growth Differ 9(3):197-208, 1998.
2. Muller J, et al. Mol Cell 8(5):983-93, 2001.
3. Sun TQ, et al. Nat Cell Biol 3(7):628-36, 2001.

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Cat# AJ1460a
(40 western blots)
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