|Application ||WB, IHC|
|Calculated MW||13157 Da|
|Other Names||Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, MLANA, MART1|
|Target/Specificity||A synthetic peptide corresponding to residues on the N-terminus of human MART-1 was used as an immungen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Precautions||MART-1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation|
|Tissue Location||Expression is restricted to melanoma and melanocyte cell lines and retina|
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Provided below are standard protocols that you may find useful for product applications.
MART-1 has been widely studied as a melanoma-specific antigen and as a melanosome-specific marker. A role for MART-1 in pigmentation is expected because its expression pattern and subcellular distribution is quite similar to the other melanosomal proteins and usually correlates with melanin content. MART-1 forms a complex with Pmel17 and affects its expression, stability, trafficking, and the processing which is required for melanosome structure and maturation. MART-1 is indispensable for Pmel17 function and thus plays an important role in regulating mammalian pigmentation (1). A gene has been identified that encodes a melanocyte lineage-specific protein (MART-1; melanoma antigen recognized by T cells 1) that is a widely shared melanoma antigen recognized by the T lymphocytes of patients with established malignancy. Identification of this gene opens possibilities for the development of immunotherapies for patients with melanoma (2).
1. Hoashi T, et al. J Biol Chem 280(14):14006-16. 2005.
2. Kawakami Y, et al. Proc Natl Acad Sci 91(9):3515-9, 1994
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