|Application ||WB, IHC|
|Calculated MW||82286 Da|
|Other Names||DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46, MCM5, CDC46|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-term of human MCM5 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MCM5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.|
|Cellular Location||Nucleus. Cytoplasm, cytosol|
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Provided below are standard protocols that you may find useful for product applications.
Human nuclear proteins MCM3 and MCM5 have high sequence similarities with the corresponding yeast proteins known to be required for the initiation of genome replication. At 0.5 M NaCl, the structure-bound nuclear protein can be partially solubilized as a dimer composed of MCM3 and the related protein MCM5. MCM3 forms stable complexes with protein MCM5. These MCM3/MCM5 complexes occur as dimers and in high-molecular-mass complexes (approximately 500 kDa). The high-molecular-mass complexes dissociate in 0.5 M NaCl and release MCM3/MCM5 dimers. It has frequently been proposed that the MCM proteins may function as licensing factors for genome replication. Data imply that the active form of an MCM protein is not a monomer, but a protein complex that includes an MCM3/MCM5 dimer (1). Levels of member of the MCM family (MCM2, MCM3, MCM5 and MCM7) gradually increased in a variable manner as KD cells progressed from GO into the G1/S phase. In the GO stage, the amounts of MCM2 and -5 proteins were much lower than those of MCM7 and -3 proteins, suggesting that they are not present in stoichiometric amounts, and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM/P1 complexes (2).
1. Burkhart R, et al. Eur J Biochem 228(2):431-8, 1995
2. Tsuruga H, et al. Biochem Biophys Res 236(1):118-25
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