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MCM7 Antibody

Rabbit Monoclonal Antibody

  • WB - MCM7 Antibody AJ1465b
    A. Western blot analysis on HeLa cell lysate using anti-MCM7 RabMAb (Cat. #AJ1465b), dilution 1:10,000.
  • IHC - MCM7 Antibody AJ1465b
    B. Immunohistochemical analysi of paraffin-embedded human lung tissue using anti-MCM7 RabMAb (Cat. #AJ1465b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P33993
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1974Y
Calculated MW 81308 Da
Gene ID 4176
Other Names DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human MCM7 was used as an immunogen.
Dilution WB~~1:2000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMCM7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MCM7
Synonyms CDC47, MCM2
Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Cellular Location Nucleus.
Citations (0)

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The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).


1. Cortez D, et al. Proc Natl Acad Sci USA 101(27):10078-83 2004
2. Fujita M, et al. J Biol Chem 271(8):4349-54, 1996
3. Tsao CC, et al. EMBO J 23(23):4660-9, 2004

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Cat# AJ1465b
(40 western blots)
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