|Application ||WB, IHC, IF|
|Calculated MW||44424 Da|
|Other Names||Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2, MEK2, MKK2, PRKMK2|
|Target/Specificity||A synthetic peptide corresponding to residues in N-terminus of human MEK2 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MEK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||MEK2, MKK2, PRKMK2|
|Function||Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).|
|Cellular Location||Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane localization is probably regulated by its interaction with KSR1|
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Provided below are standard protocols that you may find useful for product applications.
MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).
1. Crews, C. M., et al. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258: 478
2. Zheng, C.F., and K.L. Guan. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J Biol Chem. 268: 11435
3. Zheng, C.F., and K.L. Guan. Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. EMBO J. 13: 1123
4. Alessi, D.R., et al. Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1. EMBO J. 13: 1610
5. Rosen, L.B., et al. Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras. Neuron 12: 1207
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