|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||69741 Da|
|Other Names||Mitogen-activated protein kinase kinase kinase 2, MAPK/ERK kinase kinase 2, MEK kinase 2, MEKK 2, MAP3K2, MAPKKK2, MEKK2|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-term of human MEKK-2 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MEKK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.|
|Cellular Location||Cytoplasm. Nucleus. Note=Upon EGF stimulation, translocates into the nucleus|
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Provided below are standard protocols that you may find useful for product applications.
MEKK2 is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP/ERK kinase kinase family (1). Closely related to MEKK3, MEKK2 is expressed in a multitude of tissues. MEKK2 directly phosphorylates and activates Ikappa B kinases and has been found to bind and activate protein kinase C-related kinase 2 (2). MEKK2 has also been known to activate JNK1/2, ERK1/2, p38, and ERK5 MAPK in vitro (3). MEKK2 interacting protein (Mip1) regulates MEKK2 expression (4).
1. Blank, J. L., P. Gerwins, E. M. Elliott, S. Sather, and G. L. Johnson. 1996.; J. Biol. Chem. 271:5361-5368.
2. Deacon, K., and J. L. Blank. 1997. J. Biol. Chem. 272:14489-14496
3. Cheng, J., J. Yang, Y. Xia, M. Karin, and B. Su. 2000. Mol. Cell. Biol. 20:2334-2342
4. Jinke Cheng, Dongyu Zhang, Kihwan Kim, Yingxin Zhao, Yingming Zhao, Bing Su; Mol. and Cell. Biol., July 2005, p. 5955-5964, Vol. 25, No. 14
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