|Application ||WB, IHC|
|Calculated MW||69741 Da|
|Other Names||Mitogen-activated protein kinase kinase kinase 2, MAPK/ERK kinase kinase 2, MEK kinase 2, MEKK 2, MAP3K2, MAPKKK2, MEKK2|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-term of human MEKK-2 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MEKK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.|
|Cellular Location||Cytoplasm. Nucleus. Note=Upon EGF stimulation, translocates into the nucleus|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
MEKK2 is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP/ERK kinase kinase family (1). Closely related to MEKK3, MEKK2 is expressed in a multitude of tissues. MEKK2 directly phosphorylates and activates Ikappa B kinases and has been found to bind and activate protein kinase C-related kinase 2 (2). MEKK2 has also been known to activate JNK1/2, ERK1/2, p38, and ERK5 MAPK in vitro (3). MEKK2 interacting protein (Mip1) regulates MEKK2 expression (4).
1. Blank, J. L., P. Gerwins, E. M. Elliott, S. Sather, and G. L. Johnson. 1996.; J. Biol. Chem. 271:5361-5368.
2. Deacon, K., and J. L. Blank. 1997. J. Biol. Chem. 272:14489-14496
3. Cheng, J., J. Yang, Y. Xia, M. Karin, and B. Su. 2000. Mol. Cell. Biol. 20:2334-2342
4. Jinke Cheng, Dongyu Zhang, Kihwan Kim, Yingxin Zhao, Yingming Zhao, Bing Su; Mol. and Cell. Biol., July 2005, p. 5955-5964, Vol. 25, No. 14
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.