|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||70898 Da|
|Other Names||Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3, MAP3K3, MAPKKK3, MEKK3|
|Target/Specificity||A synthetic peptide corresponding to residues in the N-term of human MEKK3 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MEKK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.|
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Provided below are standard protocols that you may find useful for product applications.
MEKK3 is a Ser/Thr protein kinase belonging to the MAP/ERK kinase kinase family (1). Closely related to MEKK2, MEKK3 is expressed in a multitude of tissues. MEKK3 directly regulates SAPKs and ERKs pathway via SEK and MEK1, respectively but not the p38 pathway (2) and is also involved in the NF-kB pathway (3). MEKK3 as well as MEKK2 bind, phosphorylate and activate MEK5 through their PB1 domain (4). hKSR-2 (human kinase suppressor of ras-2) has been shown to regulate the activity of MEKK3 (5).
1. Blank, J. L., P. Gerwins, E. M. Elliott, S. Sather, and G. L. Johnson. 1996.; J. Biol. Chem. 271:5361-5368.
2. Zhang et al.: J. Biol. Chem. 272: 2668-2674, 1997.
3. Zhao, Q., and Lee, F. S. (1999) J. Biol. Chem. 274, 8355
4. Wilson , M. I., Gill, D. J., Perisic, O., Quinn, M. T., and Williams, R. L. (2003) Mol. Cell 12, 39
5. P.L. Channavajhala, L. Wu, J.W. Cuozzo, J.P. Hall, W. Liu, L.L. Lin, Y. Zhang, J. Biol. Chem. 278 (2003) 47089-47097
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