|Application ||WB, IHC|
|Calculated MW||181685 Da|
|Other Names||Mitogen-activated protein kinase kinase kinase 4, MAP three kinase 1, MAPK/ERK kinase kinase 4, MEK kinase 4, MEKK 4, MAP3K4, KIAA0213, MAPKKK4, MEKK4, MTK1|
|Target/Specificity||A synthetic peptide corresponding to residues in human MEKK4 was used as immunogen. This antibody is predicted to detect splice isoform 2 based on sequence analysis.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MEKK4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA0213, MAPKKK4, MEKK4, MTK1|
|Function||Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.|
|Cellular Location||Cytoplasm, perinuclear region. Note=Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles.|
|Tissue Location||Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.|
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Provided below are standard protocols that you may find useful for product applications.
MEK kinase 4 (MEKK4), also known as MTK1, is a Ser/Thr protein kinase belonging to the MAP kinase kinase kinase family (1). This protein contains a protein kinase catalytic domain at the C-terminus. The N-terminal, non-kinase domain may contain a regulatory domain. MEKK4 regulates p38 and JNK, butnot ERK pathway, through the phosphorylation of the MAP2Ks MKK3 and MKK6 and MKK4 and MKK7 respectively (1-2).
1. Takekawa, M.F. Posas, and H. Saito. 1997 EMBO J. 16:4973-4982
2. Gerwins, P., J.L. Blank, and G.L. Johnson. 1997. J. Biol. Chem. 272:8288-8295
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