|Application ||WB, IHC, IF|
|Calculated MW||68023 Da|
|Other Names||Menin, MEN1, SCG2|
|Target/Specificity||A synthetic peptide corresponding to residues at the C terminus of human Menin was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Menin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.|
|Cellular Location||Nucleus. Note=Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation|
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Provided below are standard protocols that you may find useful for product applications.
Menin is a nuclear protein that is ubiquitously expressed (1). Inactivating germ line mutations of this gene causes multiple endocrine neoplasia type 1 (MEN1), an inherited syndrome characterized by the combined occurrence of parathyroid adenomas, gastroenteropancreatic tumors, pituitary tumors and several other tumor types (2). Menin interacts with nuclear proteins such as transcription factors, histone methyltransferases (HMT) and histone deacetylases (HDAC) to facilitate regulation of gene transcription and cellular homeostasis. Menin interacts with a number of transcriptional factors such as JunD, NF-kB, Smad3, and homeobox-containing DNA binding protein Pem and inhibits the activities of JunD and NF-kB (3,4,5). The Menin HMT complex was found to activate several target genes, including homeobox domain (Hox) genes, cyclin-dependent kinase inhibitors tumor suppressor genes and nuclear hormone receptor responsive genes (6). Menin was demonstrated to attenuate transcription of the telomerase (hTERT) gene, possibly depending on recruitment of deacetylation activity (7).
1. Guru et al. Proc Natl Acad Sci USA 95 : 1630-4, 1998. 2. Brandi et al. J clin Endocrinol Metab 86 : 5658-71, 2001. 3. Poisson et al. Cancer letters. 189 :1-10, 2003. 4. Agarwal et al. Cell 96 : 143-152, 1999. 5. Heppner et al. Oncogene 20 : 4917-25, 2001. 6. Yang et al. Mol Cell Endocrinol 265-266 : 34-41, 2007. 7. Hashimoto et al. Int J Oncol 33 : 333-40, 2008.
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