|Calculated MW||68986 Da|
|Other Names||Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, Megakaryocyte-potentiating factor, MPF, Mesothelin, cleaved form, MSLN, MPF|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human Mesothelin was used as an immunogen. This antibody detects full-length Mesothelin precursor (69 kDa) and C-terminal cleaved form of Mesothelin (40 kDa).|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mesothelin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Membrane-anchored forms may play a role in cellular adhesion.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus Isoform 3: Secreted.|
|Tissue Location||Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).|
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Mesothelin is a tumor differentiation antigen that is present on the surface of normal mesothelial cells lining the pleura, peritoneum and pericardium. It is over expressed in many human tumors, including malignant mesothelioma and ovarian, pancreatic and lung adenocarcinomas (1). The normal biologic function of mesothelin is unknown but recent studies have shown that it forms a strong and specific complex with MUC16; a binding which has been suggested to be the basis of ovarian cancer metastasis (2). Initially, mesothelin is produced as a polypeptide with a hydrophobic tail which is removed and replaced by phosphatidylinositol. After glycosylation at the one or more of its four putative N-linked glycosylation sites, it is cleaved by a protease to yield the 40 kDa fragment (or doublet) (3). Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer (4).
1. Hassan R, et al. Eur J Cancer. 44(1):46-53, 2008 2. Sathyanarayana BK, et al. BMC Struct Biol. 9:1, 2009 3. Chang, K., et al. Proc. Natl. Acad. Sci. USA 93:136-140, 1996 4. The UniProt Consortium. The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008)
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