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Met Antibody Phospho (pY1349)

Rabbit Monoclonal Antibody

  • WB - Met Antibody Phospho (pY1349) AJ1476b
    A. Western blot analysis on A431 cell lysates using anti-Phospho-Met (pY1349) RabMAb (Cat. #AJ1476b), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP.
  • IHC - Met Antibody Phospho (pY1349) AJ1476b
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-specific anti-Met (pY1349) RabMAb (Cat. #AJ1476b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08581
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP2367Y
Calculated MW 155541 Da
Gene ID 4233
Other Names Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met, MET
Target/Specificity A phospho specific peptide corresponding to residues surrounding tyrosine 1349 of human Met was used as an immunogen. This antibody detects Met phosphorylated at tyrosine 1349.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMet Antibody Phospho (pY1349) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MET
Function Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain
Research Areas
Citations (0)

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Met is a receptor protein-tyrosine kinase (RPTK) for hepatocyte growth factor (HGF), which is a multifunctional cytokine controlling cell growth, morphogenesis, and motility. Met overexpression has been identified in a variety of human cancers (1). Met kinase domain possesses unique features that distinguish met from other members of the src family of protein tyrosine kinases. These results also demonstrate that the product of the activated met gene is a fusion protein and that the amino terminal end of this fusion protein exhibits homology to laminin B1 (2). Data suggest that RanBP9, functioning as an adaptor protein for the Met tyrosine kinase domain, can augment the HGF-Met signaling pathway and that RanBP9 overexpression may cause constitutive activation of the Ras signaling pathway (2). Interaction of the hepatocyte growth factor (HGF) with its receptor, the Met tyrosine kinase, results in invasive growth, a genetic program essential to embryonic development and implicated in tumor metastasis. Met-mediated invasive growth requires autophosphorylation of the receptor on tyrosines located in the kinase activation loop (Tyr(1234)-Tyr(1235)) and in the tail (Tyr(1349)-Tyr(1356)).


1. Wang D, et al. J Biol Chem 277(39):36216-22, 2002.
2. Chan AM, et al. Oncogene 1(2):229-33, 1987.
3. Bardelli A, et al. J Biol Chem 274(41):29274-81, 1999

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Cat# AJ1476b
(40 western blots)
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