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mGluR5 Antibody

Rabbit Monoclonal Antibody

     
  • WB - mGluR5 Antibody AJ1477a
    A. Western blot analysis on mouse brain lysate using anti-mGluR5 RabMAb (Cat. #AJ1477a), dilution 1:10,000.
  • IHC - mGluR5 Antibody AJ1477a
    B. Immunohistochemical analysis of paraffin-embedded human brain using anti-Metabotrophic Glutamate receptor 5 RabMAb (Cat. #AJ1477a).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P41594
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR2425Y
Calculated MW 132469 Da
Gene ID 2915
Other Names Metabotropic glutamate receptor 5, mGluR5, GRM5, GPRC1E, MGLUR5
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human mGluR5.
Dilution WB~~1:1000~10000
IHC~~1:250~500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsmGluR5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GRM5
Synonyms GPRC1E, MGLUR5
Function G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Cellular Location Cell membrane {ECO:0000269|PubMed:7908515, ECO:0000269|Ref.7}; Multi-pass membrane protein {ECO:0000269|PubMed:7908515, ECO:0000269|Ref.7}
Research Areas
Citations (0)

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Background

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Class I metabotropic glutamate receptors (mGluRs) have been postulated to play a role in synaptic plasticity. It has been suggested that mGluR5 may be the primary high affinity ACPD receptor in CA1 neurons (1). Also, neuroprotection by mGluR system has been linked to the modulation of both the free radical nitric oxide (NO) and programmed cell death (PCD). Activation of group I mGluR subtypes utilized an effective, upstream mechanism for the inhibition of cysteine protease activity that offered an enhanced level of neuroprotection through both the preservation of genomic DNA integrity and the maintenance of PS membrane asymmetry (2). It has also been shown that activation of mGluRs enhances formation of an mGluR-Homer-PIKE-L complex, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Our findings indicate that this complex mediates the well-known ability of agonists of mGluR5 to prevent neuronal apoptosis (3).

References

1. Lu YM, et al. J Neurosci 17(13):5196-205, 1997
2. Maiese K, et al. J Neurosci Res 62(2):257-72, 2000
3. Rong R, et al. Nat Neurosci 6(11):1153-61, 2003

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Discontinued
Cat# AJ1477a
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