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Midkine Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - Midkine Antibody (C-term) AJ1479a
    A. Western blot analysis on 1 ng of recombinant protein using anti-Midkine RabMAb (Cat. #AJ1479a), dilution 1:500.
  • IHC - Midkine Antibody (C-term) AJ1479a
    B. Immunohistochemical staining of paraffin-embedded human livercarcinoma using anti-Midkine RabMAb (Cat. #AJ1479a).
  • IF - Midkine Antibody (C-term) AJ1479a
    C. Immunofluorescent staining of HeLa cells using anti-Midkine RabMAb(Cat. #AJ1479a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21741
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1143Y
Calculated MW 15585 Da
Gene ID 4192
Other Names Midkine, MK, Amphiregulin-associated protein, ARAP, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein, MDK, MK1, NEGF2
Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human Midkine was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMidkine Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MDK
Synonyms MK1, NEGF2
Function Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity).
Cellular Location Secreted.
Tissue Location Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions
Research Areas
Citations (0)

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Midkine (MK) is a 13 kDa heparin-binding polypeptide which enhances neurite outgrowth, neuronal cell survival and plasminogen activator activity. MK is structurally divided into two domains, and most of the biological activities are located on the C-terminal domain. Both domains consist of three antiparallel beta-strands, but the C-terminal domain has a long flexible hairpin loop where a heparin-binding consensus sequence is located (1). MK is a member of a highly conserved, developmentally regulated human gene family. The gene product exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Expression of MK is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable (2).


1. Iwasaki W, et al. EMBO J 16(23):6936-46, 1997.
2. Kaname T, et al. Genomics 17(2):514-5, 1993.

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Cat# AJ1479a
(40 western blots)
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