- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
MKK4 Antibody (C-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AJ1483b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
MKK4 Antibody (C-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | P45985 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP615Y |
| Calculated MW | 44288 Da |
| Gene ID 6416 | |
| Other Names MAP2K4, JNKK1, MKK4, PRKMK4, SERK1, Dual specificity mitogen-activated protein kinase kinase 4, SAPK/ERK kinase 1 | |
| Target/Specificity A synthetic peptide corresponding to residues in the C-term of human MKK4 was used as immunogen. | |
| Dilution WB~~1:1000 IHC~~1:50~100 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions MKK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
MKK4 Antibody (C-term) - Protein Information | |
| Name MAP2K4 | |
| Synonyms JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, | |
| Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14 | |
| Cellular Location Cytoplasm (By similarity). Nucleus (By similarity) | |
| Tissue Location Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues | |
MKK4 Antibody (C-term) - Related products
AP3344a: Phospho-MAP2K4-S80 Antibody
AP7916d: MEKK4 (MAP2K4) Antibody (S257)
RI13157: MAP2K4 predesign siRNA
BP3344a: Phospho-MAP2K4-S80 (Antibody Blocking Peptide
BP7916d: MAP2K4 Antibody (S257) Blocking Peptide
MKK4 Antibody (C-term) - Application data
-
A. Western blot analysis on Hela cell lysate using anti-MKK4 (C-term) RabMAb (Cat. #AJ1483b), dilution 1:1,000.
-
B. Immunohistochemical analysis of lung adenocarcinoma using anti-MKK4 (C-term) RabMAb (Cat. #AJ1483b).
MKK4 Antibody (C-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylate and activate p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).
REFERENCES
1. Lin, et al. Science 268: 286-290, 1995
2. Sanchez I, Hughes RT, Mayer BJ, et al Nature (Lond) 1994;372:794-8
3. Lawler, S., Fleming, Y., Goedert, M., and Cohen, P. (1998) Curr. Biol. 8, 1387
4. Yoshida et al. (1999) Cancer Res. 59, 5483
