|Application ||WB, IF|
|Calculated MW||50112 Da|
|Other Names||Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MEK5, MKK5, PRKMK5|
|Target/Specificity||A synthetic peptide corresponding to residues within human MKK5 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MKK5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||MEK5, MKK5, PRKMK5|
|Function||Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.|
|Tissue Location||Expressed in many adult tissues. Abundant in heart and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
Mitogen-acitvated protein kinase kinase 5 (MKK5) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). The MAPK cascade plays an important role in signaling defense responses. Of the MKK family members, MKK5 exhibits the closest homology to MEK1 and MEK2, with 46% amino acid identity in the catalytic domain (3) MKK has been show to activate extracellular-signal-regulated protein kinase 5 (ERK5). Also, activated ERK5 phosphorylates mitogen- MKK5 extensively at Ser129, Ser137, Ser142 and Ser149, which are located within the region in MKK5 that is thought to interact with ERK5 (3). There have also been findings to that the MEK5 (MKK5)/ERK5 (BMK) pathway may play a role in prostate cancer (5).
1. Han J, et al. J Bio Chem 271:2886-2891, 1996
2. Seger R, et al. FASEB J. 9:726-735, 1995
3. Mody N, et al. FEBS Lett 502:21-24, 2001
4. Mody N, et al. J. Bio Chem 372:567-575, 2001
5. Mehta PB, et al. Oncogene 22:1381-1389, 2003
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