|Application ||WB, IF|
|Calculated MW||92688 Da|
|Other Names||Mitogen-activated protein kinase kinase kinase 11, Mixed lineage kinase 3, Src-homology 3 domain-containing proline-rich kinase, MAP3K11 (HGNC:6850)|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human MLK-3 was used as an immunogen|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MLK-3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Location is cell cycle dependent|
|Tissue Location||Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.|
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Provided below are standard protocols that you may find useful for product applications.
Mixed lineage kinase-3 (MLK-3) is a mitogen-activated kinase kinase kinase that mediates stress-activating protein kinase (SAPK)/c-Jun NH2-terminal kinase activation. MLK-3 and other MLK family kinases are characterized by the presence of multiple protein-protein interaction domains including a tandem leucine/isoleucine zipper (LZs) motif. Leucine zippers are known to mediate protein dimerization raising the possibility that the tandem leucine/isoleucine zippers may function as a dimerization motif of MLK-3 (1). MLK-3 has several interesting structural features including an SH3 domain in the absence of an SH2 domain, a region containing two leucine zippers with an adjacent carboxy-terminal basic region, and a proline rich region. (2). HPK1 has been found to phosphorylate MLK-3 activation loop in vitro, and Ser281 was found to be the major phosphorylation site, indicating that HPK1 also activates MLK-3 via phosphorylation of the kinase activation loop (3).
1. Leung IW, et al. J Biol Chem 273(49):32408-15, 1998.
2. Ing YL, et al. Oncogene 9(6):1745-50, 1994.
3. Leung IW, et al. J Biol Chem 276(3):1961-7, 2001.
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