|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||54387 Da|
|Other Names||Matrix metalloproteinase-20, MMP-20, 3424-, Enamel metalloproteinase, Enamelysin, MMP20|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human MMP-20 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MMP-20 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Cleaves aggrecan at the '360-Asn-|-Phe-361' site.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Expressed specifically in the enamel organ.|
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Provided below are standard protocols that you may find useful for product applications.
MMP-20 (enamelysin) is a recently discovered member of the MMP family. This enzyme is involved in the degradation of the various components of the extracellular matrix (ECM) during development, haemostasis and pathological conditions. MMP-20 expression has been found to be restricted to the enamel organ and due to its unique expression pattern, may primarily be involved in the turnover of these molecules during tooth development. The enamel matrix proteins amelogenin, ameloblastin, and enamelin are expressed during the developmental time period, suggesting that MMP-20 may play a role in their hydrolysis (1). Recombinant human enamelysin is able to degrade amelogenin, the major protein component of the enamel matrix. On the basis of its degrading activity on amelogenin, and its highly restricted expression to dental tissues, we suggest that human enamelysin plays a central role in the process of tooth enamel formation (2). Enamelysin null mice do not process amelogenin properly, possesses an altered enamel matrix and rod pattern, has hypoplastic enamel that delaminates from the dentin, and has a deteriorating enamel organ morphology as development progresses (3).
1. Stracke JO, et al. FEBS Lett 478(1-2):52-6, 2000.
2. Llano E, et al. Biochemistry 36(49):15101-8, 1997
3. Caterina JJ, et al. J BIol Chem 277(51):49598-604, 2002
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