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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MMP-9 Antibody   

MMP-9 Antibody

Rabbit Monoclonal Antibody

  • WB - MMP-9 Antibody AJ1503a
    A. Western blot analysis on HL-60 cell lysate using anti-MMP-9 RabMAb (Cat. #AJ1503a), dilution 1:500
  • IHC - MMP-9 Antibody AJ1503a
    B. Immunohistochemical staining of paraffin-embedded human lymphoma using anti-MMP-9 RabMAb (Cat. #AJ1503a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14780
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1255Y
Calculated MW 78458 Da
Gene ID 4318
Other Names Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, MMP9, CLG4B
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human MMP-9 was used as an immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMMP-9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMP9
Synonyms CLG4B
Function May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Produced by normal alveolar macrophages and granulocytes
Research Areas
Citations (0)

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92-kDa Type IV collagenase (MMP-9), a member of matrix metalloproteinases, is believed to play a critical role in physiological tissue-remodeling processes and also in many pathological conditions such as tumor invasion. The induction of proteolytic enzymes is an important mechanism in the migration of monocytes into tissues and body fluids (1). MMP-9 is secreted from neutrophils, macrophages, and a number of transformed cells in zymogen form (2). MMP-9/gelatinase B is upregulated in angiogenic dysplasias and invasive cancers of the epidermis in a mouse model of multi-stage tumorigenesis elicited by HPV16 oncogenes. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells (3).


1. Van Ranst M, et al. Cytokine 3(3):231-9, 1991.
2. Ogata Y, et al. J Biol Chem 267(6):3581-4, 1992.
3. Coussens LM, et al. Cell 103(3):481-90, 2000.

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Cat# AJ1503a
(40 western blots)
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