- CITATIONS: 2
|Application ||WB, IHC, IF|
|Calculated MW||78458 Da|
|Other Names||Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, MMP9, CLG4B|
|Target/Specificity||A synthetic peptide corresponding to residues on human MMP-9 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MMP-9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Produced by normal alveolar macrophages and granulocytes|
Provided below are standard protocols that you may find useful for product applications.
Matrix metalloproteinase (MMP)-9 (92-kDa Type IV collagenase) belongs to the MMP family of zinc-dependent endopeptidases that has been associated with tumor cell invasion and metastasis and tumor-induced angiogenesis. As a secreted MMP, pro-MMP-9 is released into the extracellular environment by both tumor and stroma cells, where it fulfills its proteolytic functions degrading both extracellular matrix (ECM) and non-ECM proteins (1). MMP-9 is also reported to have proteolytic activity against connective tissue proteins, and has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture (2). MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells and has been found to be involved in a variety of autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, systemic sclerosis, rheumatoid arthritis, multiple sclerosis, polymyositis and atherosclerosis. It plays either a primary or secondary role in each one of those autoimmune diseases by its up or down-regulation, making it a target for therapy of autoimmune diseases (3, 4).
1. Fridman R, et al. Cancer Metastasis Rev. 22(2-3):153-66,2003.
2. Zhang B, et al. Circulation. 99(14):1788-94, 1999.
3. Coussens LM, et al. Cell 103(3):481-90, 2000.
4. Ram M, et al. J Clin Immunol. 26(4):299-307, 2006
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