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MSH2 Antibody

Rabbit Monoclonal IgG

  • WB - MSH2 Antibody AJ1509b
    Western blot analysis on (A) K562, (B) HeLa, and (C) A375 cell lysates using anti-MSH2 RabMAb (Cat. #AJ1509b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P43246
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3944
Calculated MW 104743 Da
Gene ID 4436
Other Names DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2
Target/Specificity A synthetic peptide corresponding to residues in human MSH2 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMSH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MSH2
Function Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Cellular Location Nucleus.
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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MutS homologue 2 (MSH2) is a DNA mismatch repair protein in the MutS family (1). MSH2 forms two different heterodimers: MutS alpha (MSH2-MSH6) and MutS beta (MSH2-MSH3) that bind to DNA mismatches thereby initiating DNA repair. (2). MutS alpha bends the DNA helix and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA (2). MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long (2). Heterozygous mutations in the MSH2 gene are a cause of hereditary nonpolyposis colorectal cancer (HNPCC), forming a specific mispair binding complex with MSH3 and MSH6 (3, 4). MSH2 is the most frequently detected mutation among MutS family proteins in HNPCC (1). MutS alpha may also play a role in DNA homologous recombination repair (2).


1. Online Mendelian Inheritance in Man, OMIM (TM). Johns Hopkins University, Baltimore, MD. MIM Number: {600678}: {4/7/2009}: . World Wide Web URL: 2. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2009). 3. Guerrette et al. Mol Cell Biol. 1998 Nov;18(11):6616-23. 4. Wimmer et al. Hum Genet. 124(2):105-22, 2008.

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Cat# AJ1509b
(40 western blots)
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