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MSH6 Antibody

Rabbit Monoclonal IgG

  • WB - MSH6 Antibody AJ1510a
    Western blot analysis on (A) A431, (B) HeLa, and (C) SW480 cell lysates using anti-MSH6 RabMAb (Cat. #AJ1510a).
  • IHC - MSH6 Antibody AJ1510a
    Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using anti-MSH6 RabMAb (Cat. #AJ1510a).
  • IF - MSH6 Antibody AJ1510a
    Immunofluorescent staining of HeLa cells using anti-MSH6 RabMAb (Cat. #AJ1510a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P52701
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EPR3945
Calculated MW 152786 Da
Gene ID 2956
Other Names DNA mismatch repair protein Msh6, hMSH6, G/T mismatch-binding protein, GTBP, GTMBP, MutS-alpha 160 kDa subunit, p160, MSH6, GTBP
Target/Specificity A synthetic peptide corresponding to residues at the N-terminus in human MSH6 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMSH6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MSH6
Synonyms GTBP
Function Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
Cellular Location Nucleus. Chromosome. Note=Associates with H3K36me3 via its PWWP domain
Citations (0)

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The MutS homologue 6 protein (MSH6) is a member of the MutS homolog family required in the DNA mismatch repair system (1). MSH6 forms a MutS alpha dimer with MSH2, binding to DNA mismatches to initiate DNA repair (2). MutS alpha bends the DNA helix and recognizes single base mismatches and dinucleotide insertion-deletion loops in the DNA (2). Heterozygous mutations in the MSH6 gene are a cause of hereditary nonpolyposis colorectal cancer (HNPCC), forming a specific mispair binding complex with MSH3 (3, 4). The frequency of MSH6 mutation is higher in HNPCC than in atypical HNPCC (5). The MSH2/MSH6 dimer may also play a role in DNA homologous recombination repair (2).


1. Online Mendelian Inheritance in Man, OMIM (TM). Johns Hopkins University, Baltimore, MD. MIM Number: {600678}: {4/7/2009}: . World Wide Web URL: 2. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2009) 3. Guerrette et al. Mol Cell Biol. 1998 Nov;18(11):6616-23. 4. Wimmer et al. Hum Genet. 2008 Sep;124(2):105-22. 5. Zhao et al. J Toxicol Environ Health A. 72(11):690-7, 2009.

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Cat# AJ1510a
(40 western blots)
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