|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||18789 Da|
|Other Names||Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, MLC-2, MLC-2v, MYL2|
|Target/Specificity||A synthetic peptide corresponding to residues in human Myosin Light Chain 2 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Myosin Light Chain 2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Cytoplasm, myofibril, sarcomere, A band|
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Provided below are standard protocols that you may find useful for product applications.
Myosin is a hexamer of 2 identical heavy chains and 2 pairs of light chains (1). The two pairs of light chains of muscle myosins are called essential light chains (ELC) and regulatory light chains (RLC). The light chains stabilize the long alpha helical neck of the myosin head (2). Myosin light chain-2 (MYL2), also known as the regulatory light chain of myosin, is an important protein involved in the regulation of myosin ATPase activity in smooth muscle (3). Calcium triggers the phosphorylation of MYL2 that in turn triggers contraction. The phosphorylation sites of MYL2 are mainly Serine 19 (monophosphorylation) and, in some conditions, both Threonine 18 and Serine 19 (diphosphorylation) (4). An increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis (3).
1. Sweeney, Biophys J. 1995; 68:112s-119s. 2. Poetter K, et al., Nat Genet. 13(1):63-9, 1996 3. Macera MJ, et al. Genomics. 13(3):829-31,1992 4. Ikebe et al, Biochemistry 1989; 28: 8750-8755.
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