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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Myosin Light Chain 2 Antibody   

Myosin Light Chain 2 Antibody

Rabbit Monoclonal Antibody

  • WB - Myosin Light Chain 2 Antibody AJ1521a
    Western blot analysis on (A) fetal heart, (B) fetal skeletal muscle, (C) human heart, (D) mouse heart and (E) rat heart lysates using anti-Myosin Light Chain 2 RabMAb (Cat. #AJ1521a).
  • IHC - Myosin Light Chain 2 Antibody AJ1521a
    Immunohistochemical analysis of paraffin-embedded human heart muscle using anti-Myosin Light Chain 2 RabMAb (Cat. #AJ1521a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10916
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3741
Calculated MW 18789 Da
Gene ID 4633
Other Names Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, MLC-2, MLC-2v, MYL2
Target/Specificity A synthetic peptide corresponding to residues in human Myosin Light Chain 2 was used as an immunogen.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyosin Light Chain 2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYL2 (HGNC:7583)
Function Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
Cellular Location Cytoplasm, myofibril, sarcomere, A band {ECO:0000250|UniProtKB:P08733}
Research Areas
Citations (0)

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Myosin is a hexamer of 2 identical heavy chains and 2 pairs of light chains (1). The two pairs of light chains of muscle myosins are called essential light chains (ELC) and regulatory light chains (RLC). The light chains stabilize the long alpha helical neck of the myosin head (2). Myosin light chain-2 (MYL2), also known as the regulatory light chain of myosin, is an important protein involved in the regulation of myosin ATPase activity in smooth muscle (3). Calcium triggers the phosphorylation of MYL2 that in turn triggers contraction. The phosphorylation sites of MYL2 are mainly Serine 19 (monophosphorylation) and, in some conditions, both Threonine 18 and Serine 19 (diphosphorylation) (4). An increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis (3).


1. Sweeney, Biophys J. 1995; 68:112s-119s. 2. Poetter K, et al., Nat Genet. 13(1):63-9, 1996 3. Macera MJ, et al. Genomics. 13(3):829-31,1992 4. Ikebe et al, Biochemistry 1989; 28: 8750-8755.

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Cat# AJ1521a
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