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MYPT1 Antibody (N-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1523a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
MYPT1 Antibody (N-term) - Product info | |
| Application | WB
|
| Primary Accession | O14974 |
| Reactivity | Human |
| Clone Names | Y110 |
| Calculated MW | 115281 Da |
| Gene ID 4659 | |
| Other Names PPP1R12A, MBS, MYPT1, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1;Myosin phosphatase target subunit 1;Protein phosphatase myosin-binding subunit | |
| Target/Specificity A synthetic peptide corresponding to residues in the first ANK repeat of human MYPT1 was used as an immunogen. This antibody can detect all 4 splice isoforms of MYPT1. | |
| Dilution WB~~1:1000~10000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions MYPT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
MYPT1 Antibody (N-term) - Protein Information | |
| Name PPP1R12A | |
| Synonyms MBS, MYPT1 | |
| Function Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity | |
| Cellular Location Cytoplasm. Note=Along actomyosin filaments and stress fibers | |
| Tissue Location Expressed in striated muscles, specifically in type 2a fibers (at protein level) | |
MYPT1 Antibody (N-term) - Related products
AP9667b: PPP1R12A Antibody (C-term)
BP9667b: PPP1R12A Antibody (C-term) Blocking Peptide
MYPT1 Antibody (N-term) - Application data
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A. Western blot analysis on 293 cell lysate using anti-MYPT1 RabMAb (Cat. #AJ1523a).
MYPT1 Antibody (N-term) - Research Areas
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BACKGROUND
The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component act as a regulatory subunit known as myosin binding (MBS) or myosin phosphatase targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoforms of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.
REFERENCES
1. Alessi, D., MacDougall, L. K., Sola, M. M., Ikebe, M., and Cohen, P.(1992) Eur. J. Biochem. 210, 1023-1035
2. Fujioka, M.; Takahashi, N.; Odai, H.; Araki, S.; Ichikawa, K.;Feng, J.; Nakamura, M.; Kaibuchi, K.; Hartshorne, D. J.; Nakano, T.;Ito, M. Genomics 49: 59-68, 1998.
3. Hartshorne, D. J., Ito, M., and Erdodi, F. J. Muscle Res. Cell Motil. 19, 325-341, (1998)
4. Arimura T., Suematsu N., Zhou Y.-B., Nishimura J., Satoh S., Takeshita A., Kanaide H., Kimura A.; J. Biol. Chem. 276:6073-6082(2001).
