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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MYPT1/MYPT2 Antibody (C-term)   

MYPT1/MYPT2 Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - MYPT1/MYPT2 Antibody (C-term) AJ1523b
    A. Western blot analysis on 293T cell lysate using anti-MYPT1/MYPT2 RabMAb (Cat. #AJ1523b), dilution 1:5,000.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O14974
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names YE336
Calculated MW 115281 Da
Gene ID 4659
Other Names Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit, PPP1R12A, MBS, MYPT1
Target/Specificity A synthetic peptide corresponding to residues in C-terminus of human MYPT1 or MYPT2 was used as immunogen.
Dilution WB~~1:5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYPT1/MYPT2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP1R12A
Synonyms MBS, MYPT1
Function Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity.
Cellular Location Cytoplasm. Note=Along actomyosin filaments and stress fibers
Tissue Location Expressed in striated muscles, specifically in type 2a fibers (at protein level).
Research Areas
Citations (0)

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The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component acts as a regulatory subunit known as myosin binding (MBS) or myosin phosphatase targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoforms of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.


1. Alessi, D., MacDougall, L. K., Sola, M. M., Ikebe, M., and Cohen, P.(1992) Eur. J. Biochem. 210, 1023-1035
2. Fujioka, M.; Takahashi, N.; Odai, H.; Araki, S.; Ichikawa, K.;Feng, J.; Nakamura, M.; Kaibuchi, K.; Hartshorne, D. J.; Nakano, T.;Ito, M. Genomics 49: 59-68, 1998.
3. Hartshorne, D. J., Ito, M., and Erdodi, F. J. Muscle Res. Cell Motil. 19, 325-341, (1998)
4. Arimura T., Suematsu N., Zhou Y.-B., Nishimura J., Satoh S., Takeshita A., Kanaide H., Kimura A.; J. Biol. Chem. 276:6073-6082(2001).

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Cat# AJ1523b
(40 western blots)
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