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MYPT1/MYPT2 Antibody (C-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1523b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
MYPT1/MYPT2 Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | O14974 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | YE336 |
| Calculated MW | 115281 Da |
| Gene ID 4659 | |
| Target/Specificity A synthetic peptide corresponding to residues in C-terminus of human MYPT1 or MYPT2 was used as immunogen. | |
| Dilution WB~~1:5000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions MYPT1/MYPT2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
MYPT1/MYPT2 Antibody (C-term) - Protein Information | |
| Name PPP1R12A | |
| Synonyms MBS, MYPT1 | |
| Function Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity | |
| Cellular Location Cytoplasm. Note=Along actomyosin filaments and stress fibers | |
| Tissue Location Expressed in striated muscles, specifically in type 2a fibers (at protein level) | |
MYPT1/MYPT2 Antibody (C-term) - Related products
AP9667b: PPP1R12A Antibody (C-term)
BP9667b: PPP1R12A Antibody (C-term) Blocking Peptide
MYPT1/MYPT2 Antibody (C-term) - Application data
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A. Western blot analysis on 293T cell lysate using anti-MYPT1/MYPT2 RabMAb (Cat. #AJ1523b), dilution 1:5,000.
MYPT1/MYPT2 Antibody (C-term) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component acts as a regulatory subunit known as myosin binding (MBS) or myosin phosphatase targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoforms of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.
REFERENCES
1. Alessi, D., MacDougall, L. K., Sola, M. M., Ikebe, M., and Cohen, P.(1992) Eur. J. Biochem. 210, 1023-1035
2. Fujioka, M.; Takahashi, N.; Odai, H.; Araki, S.; Ichikawa, K.;Feng, J.; Nakamura, M.; Kaibuchi, K.; Hartshorne, D. J.; Nakano, T.;Ito, M. Genomics 49: 59-68, 1998.
3. Hartshorne, D. J., Ito, M., and Erdodi, F. J. Muscle Res. Cell Motil. 19, 325-341, (1998)
4. Arimura T., Suematsu N., Zhou Y.-B., Nishimura J., Satoh S., Takeshita A., Kanaide H., Kimura A.; J. Biol. Chem. 276:6073-6082(2001).
